Article
Engineering an enhanced, thermostable, monomeric bacterial luciferase gene as a reporter in plant protoplasts.
PloS one - 1 Jan 2014
Cui Boyu, Zhang Lifeng, Song Yunhong, Wei Jinsong, Li Changfu, Wang Tietao, Wang Yao, Zhao Tianyong, Shen Xihui
Abstract excerpt
The application of the luxCDABE operon of the bioluminescent bacterium Photorhabdus luminescens as a reporter has been published for bacteria, yeast and mammalian cells. We report here the optimization of fused luxAB (the bacterial luciferase heterodimeric enzyme) expression, quantum yield and its application as a reporter gene in plant protoplasts. The fused luxAB gene was mutated by error prone PCR or chemical...
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