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Article

Protein engineering of toluene-o-xylene monooxygenase from Pseudomonas stutzeri OX1 for synthesizing 4-methylresorcinol, methylhydroquinone, and pyrogallol.

Applied and environmental microbiology - 1 Jun 2004

Vardar Gönül, Wood Thomas K

Abstract excerpt

Toluene-o-xylene monooxygenase (ToMO) from Pseudomonas stutzeri OX1 oxidizes toluene to 3- and 4-methylcatechol and oxidizes benzene to form phenol; in this study ToMO was found to also form catechol and 1,2,3-trihydroxybenzene (1,2,3-THB) from phenol. To synthesize novel dihydroxy and trihydroxy derivatives of benzene and toluene, DNA shuffling of the alpha-hydroxylase fragment of ToMO (TouA) and saturation...

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Identifiers

PMID: 15184119
DOI: 10.1128/AEM.70.6.3253-3262.2004
PMCID: PMC427803

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Source receipt: pubmed, retrieved 7 Sept 2026.

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