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Article

Bimodal activation of BubR1 by Bub3 sustains mitotic checkpoint signaling.

Proceedings of the National Academy of Sciences of the United States of America - 7 Oct 2014

Han Joo Seok, Vitre Benjamin, Fachinetti Daniele, Cleveland Don W

Abstract excerpt

The mitotic checkpoint (also known as the spindle assembly checkpoint) prevents premature anaphase onset through generation of an inhibitor of the E3 ubiquitin ligase APC/C, whose ubiquitination of cyclin B and securin targets them for degradation. Combining in vitro reconstitution and cell-based assays, we now identify dual mechanisms through which Bub3 promotes mitotic checkpoint signaling. Bub3 enhances...

Topics

Anaphase-Promoting Complex-Cyclosome
Binding Sites
Cdc20 Proteins
Cell Cycle Proteins
Cell Line, Tumor
Cyclin B1
Fluorescence Recovery After Photobleaching
Green Fluorescent Proteins
HeLa Cells
Humans

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Kinetochores

M Phase Cell Cycle Checkpoints

Microscopy, Fluorescence

Mutation

Poly-ADP-Ribose Binding Proteins

Protein Binding

Protein Serine-Threonine Kinases

RNA Interference

Source

Open in PubMed Open full text

Identifiers

PMID: 25246557
DOI: 10.1073/pnas.1416277111
PMCID: PMC4210015

Genes

APC

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-25246557-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Bimodal activation of BubR1 by Bub3 sustains mitotic checkpoint signaling.PMID 25246557Read ↗

The Bub1-Plk1 kinase complex promotes spindle checkpoint signalling through Cdc20 phosphorylation.Semantic proximity Read ↗

BUB1 and BUBR1: multifaceted kinases of the cell cycle.Semantic proximity Read ↗

Recruitment of Cdc20 to the kinetochore requires BubR1 but not Mad2 in Drosophila melanogaster.Semantic proximity Read ↗

BubR1 recruitment to the kinetochore via Bub1 enhances Spindle Assembly Checkpoint signalingSemantic proximity Read ↗

The internal Cdc20 binding site in BubR1 facilitates both spindle assembly checkpoint signalling and silencing.Semantic proximity Read ↗

Saccharomyces cerevisiae BUB2 prevents mitotic exit in response to both spindle and kinetochore damage.Semantic proximity Read ↗

The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C).Semantic proximity Read ↗

Two complexes of spindle checkpoint proteins containing Cdc20 and Mad2 assemble during mitosis independently of the kinetochore in Saccharomyces cerevisiae.Semantic proximity Read ↗

BubR1 kinase: protection against aneuploidy and premature aging.Semantic proximity Read ↗

Uncoupling of the spindle-checkpoint and chromosome-congression functions of BubR1.Semantic proximity Read ↗

The Mitotic Checkpoint Complex Requires an Evolutionary Conserved Cassette to Bind and Inhibit Active APC/C.Semantic proximity Read ↗

Mad3p, a pseudosubstrate inhibitor of APCCdc20 in the spindle assembly checkpoint.Semantic proximity Read ↗

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