Article
In vivo single-molecule imaging identifies altered dynamics of calcium channels in dystrophin-mutant C. elegans.
Nature communications - 18 Sept 2014
Zhan Hong, Stanciauskas Ramunas, Stigloher Christian, Keomanee-Dizon Kevin, Jospin Maelle, Bessereau Jean-Louis, Pinaud Fabien
Abstract excerpt
Single-molecule (SM) fluorescence microscopy allows the imaging of biomolecules in cultured cells with a precision of a few nanometres but has yet to be implemented in living adult animals. Here we used split-GFP (green fluorescent protein) fusions and complementation-activated light microscopy (CALM) for subresolution imaging of individual membrane proteins in live Caenorhabditis elegans (C. elegans). In vivo...
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