Article
Substrate binding and specificity of rhomboid intramembrane protease revealed by substrate-peptide complex structures.
The EMBO journal - 16 Oct 2014
Zoll Sebastian, Stanchev Stancho, Began Jakub, Skerle Jan, Lepšík Martin, Peclinovská Lucie, Majer Pavel, Strisovsky Kvido
Abstract excerpt
The mechanisms of intramembrane proteases are incompletely understood due to the lack of structural data on substrate complexes. To gain insight into substrate binding by rhomboid proteases, we have synthesised a series of novel peptidyl-chloromethylketone (CMK) inhibitors and analysed their interactions with Escherichia coli rhomboid GlpG enzymologically and structurally. We show that peptidyl-CMKs derived from...
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