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Improved Serum- and Feeder-Free Culture of Mouse Germline Stem Cells1

11 Sept 2014

Abstract excerpt

Spermatogonial stem cells (SSCs) undergo self-renewal division, which can be recapitulated in vitro. Attempts to establish serum-free culture conditions for SSCs have met with limited success. Although we previously reported that SSCs can be cultured without serum on laminin-coated plates, the growth rate and SSC concentration were relatively low, which made it inefficient for culturing large numbers of SSCs. In...

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PMID: 25210127
DOI: 10.1095/biolreprod.114.122317

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