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Serum- and Feeder-Free Culture of Mouse Germline Stem Cells1

16 Sept 2010

Abstract excerpt

Spermatogonial stem cells (SSCs) undergo self-renewal divisions to support spermatogenesis. Although several in vitro SSC culture systems have been developed, these systems include serum or fibroblast feeders, which complicate SSC self-renewal analyses. Here, we developed a serum- and feeder-free culture system for long-term propagation of SSCs. In addition to the SSC self-renewal factors, including glial cell...

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PMID: 20844279
DOI: 10.1095/biolreprod.110.086462

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