Article
Substrate binding activates the designed triple mutant of the colicin E7 metallonuclease.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry - 1 Dec 2014
Németh Eszter, Körtvélyesi Tamás, Kožíšek Milan, Thulstrup Peter W, Christensen Hans E M, Asaka Masamitsu N, Nagata Kyosuke, Gyurcsik Béla
Abstract excerpt
The nuclease domain of colicin E7 (NColE7) cleaves DNA nonspecifically. The active center is a Zn(2+)-containing HNH motif at the C-terminus. The N-terminal loop is essential for the catalytic activity providing opportunity for allosteric modulation of the enzyme. To identify the key residues responsible for the structural integrity of NColE7, a virtual alanine scan was performed on a semiempirical quantum...
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