Article
Importance of the positive charge cluster in Escherichia coli ribonuclease HI for the effective binding of the substrate.
The Journal of biological chemistry - 25 Jun 1991
Kanaya S, Katsuda-Nakai C, Ikehara M
Abstract excerpt
The handle region (residues 84-99) in ribonuclease HI (RNase HI) from Escherichia coli, which is rich in basic amino acid residues, was altered by alanine-scanning mutagenesis. Fifteen mutant proteins were purified to homogeneity and analyzed for the enzymatic activity. A mutation of either of 2...
Topics
- Amino Acid Sequence
- Base Sequence
- Circular Dichroism
- Endoribonucleases
- Escherichia coli
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Plasmids
- Protein Conformation
- Ribonuclease H
- Substrate Specificity
- Tryptophan