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Article

Importance of the positive charge cluster in Escherichia coli ribonuclease HI for the effective binding of the substrate.

The Journal of biological chemistry - 25 Jun 1991

Kanaya S, Katsuda-Nakai C, Ikehara M

Abstract excerpt

The handle region (residues 84-99) in ribonuclease HI (RNase HI) from Escherichia coli, which is rich in basic amino acid residues, was altered by alanine-scanning mutagenesis. Fifteen mutant proteins were purified to homogeneity and analyzed for the enzymatic activity. A mutation of either of 2 tryptophan residues at 85 or 90 resulted in a large increase in the Km value along with a large decrease in the Vmax...

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PMID: 1646812

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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