Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Crystal structural analysis and metal-dependent stability and activity studies of the ColE7 endonuclease domain in complex with DNA/Zn2+ or inhibitor/Ni2+.

Protein science : a publication of the Protein Society - 1 Feb 2006

Doudeva Lyudmila G, Huang Hsinchin, Hsia Kuo-Chiang, Shi Zhonghao, Li Chia-Lung, Shen Yongliang, Cheng Yi-Sheng, Yuan Hanna S

Abstract excerpt

The nuclease domain of ColE7 (N-ColE7) contains an H-N-H motif that folds in a beta beta alpha-metal topology. Here we report the crystal structures of a Zn2+-bound N-ColE7 (H545E mutant) in complex with a 12-bp duplex DNA and a Ni2+-bound N-ColE7 in complex with the inhibitor Im7 at a resolution of 2.5 A and 2.0 A, respectively. Metal-dependent cleavage assays showed that N-ColE7 cleaves double-stranded DNA with...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Motifs
Share to Cations, Divalent

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Colicins

Share to Crystallography, X-Ray

Share to Enzyme Stability

Share to Histidine

Share to Nickel

Source

Open in PubMed Open full text

Identifiers

PMID: 16434744
DOI: 10.1110/ps.051903406
PMCID: PMC2242460

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Crystal structural analysis and metal-dependent stability and activity studies of the ColE7 endonuclease domain in complex with DNA/Zn2+ or inhibitor/Ni2+.PMID 16434744Read ↗

The conserved asparagine in the HNH motif serves an important structural role in metal finger endonucleases.Semantic proximity Read ↗

The zinc ion in the HNH motif of the endonuclease domain of colicin E7 is not required for DNA binding but is essential for DNA hydrolysis.Semantic proximity Read ↗

Dual role for Zn2+ in maintaining structural integrity and inducing DNA sequence specificity in a promiscuous endonuclease.Semantic proximity Read ↗

Increasing cleavage specificity and activity of restriction endonuclease KpnI.Semantic proximity Read ↗

Two distinct modes of metal ion binding in the nuclease active site of a viral DNA-packaging terminase: insight into the two-metal-ion catalytic mechanism.Semantic proximity Read ↗

Second-Shell Basic Residues Expand the Two-Metal-Ion Architecture of DNA and RNA Processing Enzymes.Semantic proximity Read ↗

DNA cleavage by EcoRV endonuclease: two metal ions in three metal ion binding sites.Semantic proximity Read ↗

Structural integrity of the beta beta alpha-Metal finger motif is required for DNA binding and stable protein-DNA complex formation in R.KpnI.Semantic proximity Read ↗

Structure and mechanism of the 2',3' phosphatase component of the bacterial Pnkp-Hen1 RNA repair system.Semantic proximity Read ↗

Mechanistic Studies Reveal Similar Catalytic Strategies for Phosphodiester Bond Hydrolysis by Protein-only and RNA-dependent Ribonuclease P.Semantic proximity Read ↗

Substrate binding activates the designed triple mutant of the colicin E7 metallonuclease.Semantic proximity Read ↗

The Tn7 transposase is a heteromeric complex in which DNA breakage and joining activities are distributed between different gene products.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.