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Article

Structural basis of an engineered dual-specific antibody: conformational diversity leads to a hypervariable loop metal-binding site.

Protein engineering, design & selection : PEDS - 1 Oct 2014

Fanning Sean W, Walter Richard, Horn James R

Abstract excerpt

To explore dual-specificity in a small protein interface, we previously generated a 'metal switch' anti-RNase A VHH antibody using a combinatorial histidine library approach. While most metal-binding sites in proteins are found within rigid secondary structure, the engineered VHH antibody (VHH(metal)), which contained three new histidine residues, possessed metal-binding residues within the flexible hypervariable...

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Identifiers

PMID: 25143596
DOI: 10.1093/protein/gzu033

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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