Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Quantitative assessment on the cloning efficiencies of lentiviral transfer vectors with a unique clone site.

Scientific reports - 1 Jan 2012

Zhang Gang, Tandon Anurag

Abstract excerpt

Lentiviral vectors (LVs) are powerful tools for transgene expression in vivo and in vitro. However, the construction of LVs is of low efficiency, due to the large sizes and lack of proper clone sites. Therefore, it is critical to develop efficient strategies for cloning LVs. Here, we reported a c...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Deoxyribonuclease BamHI
Share to Deoxyribonucleases, Type II Site-Specific
Share to Electrophoresis, Agar Gel

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genetic Vectors

Share to Green Fluorescent Proteins

Share to Lentivirus

Share to Microscopy, Fluorescence

Share to Transgenes

Source

Open in PubMed Open full text

Identifiers

PMID: 22629482
DOI: 10.1038/srep00415
PMCID: PMC3357496

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-22629482-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Quantitative assessment on the cloning efficiencies of lentiviral transfer vectors with a unique clone site.PMID 22629482Read ↗

Efficient lentiviral transduction of different human and mouse cellsSemantic proximity Read ↗

Lentiviral vector packaging and producer cell lines yield titers equivalent to the industry-standard four-plasmid processSemantic proximity Read ↗

Recent advances in lentiviral vector development and applications.Semantic proximity Read ↗

[A method for introducing mutations into large vectors].Semantic proximity Read ↗

The Era of Gene Therapy: The Advancement of Lentiviral Vectors and its PseudotypingSemantic proximity Read ↗

New developments in lentiviral vector design, production and purificationSemantic proximity Read ↗

Impact of the size of CRISPR-Cas gene cassettes on the packaging efficiency and transduction titer of lentiviral vectorsSemantic proximity Read ↗

The Inside Out of Lentiviral VectorsSemantic proximity Read ↗

Optimized production and concentration of lentiviral vectors containing large insertsSemantic proximity Read ↗

Production of lentiviral vectorsSemantic proximity Read ↗

Development of Lentiviral Packaging Cells and Scale Up of Production to Meet the Growing Demand in Cell and Gene TherapySemantic proximity Read ↗

P3 site-directed mutagenesis: An efficient method based on primer pairs with 3'-overhangs.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.