Article
Detection of mutations in myeloid malignancies through paired-sample analysis of microdroplet-PCR deep sequencing data.
The Journal of molecular diagnostics : JMD - 1 Sept 2014
Cheng Donavan T, Cheng Janice, Mitchell Talia N, Syed Aijazuddin, Zehir Ahmet, Mensah Nana Yaa T, Oultache Alifya, Nafa Khedoudja, Levine Ross L, Arcila Maria E, Berger Michael F, Hedvat Cyrus V
Abstract excerpt
Amplicon-based methods for targeted resequencing of cancer genes have gained traction in the clinic as a strategy for molecular diagnostic testing. An 847-amplicon panel was designed with the RainDance DeepSeq system, covering most exons of 28 genes relevant to acute myeloid leukemia and myeloproliferative neoplasms. We developed a paired-sample analysis pipeline for variant calling and sought to assess its...
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