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Article

Folding LacZ in the periplasm of Escherichia coli.

Journal of bacteriology - 1 Sept 2014

Dwyer Robert S, Malinverni Juliana C, Boyd Dana, Beckwith Jon, Silhavy Thomas J

Abstract excerpt

Targeted, translational LacZ fusions provided the initial support for the signal sequence hypothesis in prokaryotes and allowed for selection of the mutations that identified the Sec translocon. Many of these selections relied on the fact that expression of targeted, translational lacZ fusions like malE-lacZ and lamB-lacZ42-1 causes lethal toxicity as folded LacZ jams the translocation pore. However, there is...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 25002543
DOI: 10.1128/JB.01843-14
PMCID: PMC4135689

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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