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Characterization of Phytoene Desaturase Knockout Carotenoid-Deficient Microalgal Mutants Generated by Cas9-Ribonucleoprotein Complexes.

Physiologia plantarum - 1 Jan 2026

Molina-Márquez Ana, Kelterborn Simon, Hegemann Peter, Pérez-Rodríguez Miguel, Vigara Javier, León Rosa

Abstract excerpt

Phytoene desaturase (PDS; EC 1.3.5.5) is a key enzyme of the carotenoid biosynthetic pathway, catalyzing the desaturation of phytoene, precursor of all carotenoids. In this study, several PDS-knockout (PDS-KO) transformants of the chlorophyte microalga Chlamydomonas reinhardtii were generated using a reverse genetics strategy. Two single guide RNAs (sgRNA) were designed to target the first exon of the PDS gene,...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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PMID: 41757451
DOI: 10.1111/ppl.70811
PMCID: PMC12947057

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