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Article

Monitoring protein misfolding by site-specific labeling of proteins in vivo.

PloS one - 1 Jan 2014

Hsieh Tzung-yang, Nillegoda Nadinath B, Tyedmers Jens, Bukau Bernd, Mogk Axel, Kramer Günter

Abstract excerpt

Incorporating fluorescent amino acids by suppression of the TAG amber codon is a useful tool for site-specific labeling of proteins and visualizing their localization in living cells. Here we use a plasmid encoded orthogonal tRNA/aminoacyl-tRNA synthetase pair to site-specifically label firefly luciferase with the environmentally sensitive fluorescent amino acid, 3-(6-acetylnaphthalen-2-ylamino)-2- aminopropanoic...

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Source

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Identifiers

PMID: 24915041
DOI: 10.1371/journal.pone.0099395
PMCID: PMC4051779

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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