Acidic amino acids in the first intracellular loop contribute to voltage- and calcium- dependent gating of anoctamin1/TMEM16A.

doi:10.1371/journal.pone.0099376

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Article

Acidic amino acids in the first intracellular loop contribute to voltage- and calcium- dependent gating of anoctamin1/TMEM16A.

PloS one - 1 Jan 2014

Xiao Qinghuan, Cui Yuanyuan

Abstract excerpt

Anoctamin1 (Ano1, or TMEM16A) is a Ca2+-activated chloride channel that is gated by both voltage and Ca2+. We have previously identified that the first intracellular loop that contains a high density of acidic residues mediates voltage- and calcium-dependent gating of Ano1. Mutation of the four consecutive glutamates (444EEEE447) inhibits the voltage-dependent activation of Ano1, whereas deletion of these...

Topics

Action Potentials
Amino Acid Sequence
Amino Acids, Acidic
Anoctamin-1
Calcium
Chloride Channels
HEK293 Cells
Humans
Kinetics
Mutation
Neoplasm Proteins
Patch-Clamp Techniques

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Identifiers

PMID: 24901998
DOI: 10.1371/journal.pone.0099376
PMCID: PMC4047086

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