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Article

Characterization of a computationally designed water-soluble human μ-opioid receptor variant using available structural information.

Anesthesiology - 1 Oct 2014

Zhao Xuelian, Perez-Aguilar Jose Manuel, Matsunaga Felipe, Lerner Mitchell, Xi Jin, Selling Bernard, Johnson A T Charlie, Saven Jeffery G, Liu Renyu

Abstract excerpt

BACKGROUND: The recent X-ray crystal structure of the murine μ-opioid receptor (MUR) allowed the authors to reengineer a previously designed water-soluble variant of the transmembrane portion of the human MUR (wsMUR-TM). METHODS: The new variant of water-soluble MUR (wsMUR-TM_v2) was engineered based on the murine MUR crystal structure. This novel variant was expressed in Escherichia coli and purified. The...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 24835677
DOI: 10.1097/ALN.0000000000000308
PMCID: PMC4175066

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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