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Article

Construction and characterization of a kappa opioid receptor devoid of all free cysteines.

Protein engineering, design & selection : PEDS - 1 Jan 2004

Ott David, Frischknecht Renato, Plückthun Andreas

Abstract excerpt

We have constructed an optimized mutant of the kappa opioid receptor (KOR), which is devoid of its 10 free cysteines. It was necessary to test different amino acid replacements at various positions and we used a structural model and homology with other receptor family members as a guide. This mutant binds ligands and couples to the cognate G-proteins in a very similar fashion to wild-type KOR. The addition of the...

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Source

Identifiers

PMID: 14985536
DOI: 10.1093/protein/gzh004

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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