Article
Immunotoxin mediated killing of clonable T-lymphocytes infiltrating an irreversibly rejected human renal allograft.
Journal of biological regulators and homeostatic agents - 1 Jan 2000
Siena S, Bregni M, Formosa A, Brando B, Lappi D A, Bonadonna G, Gianni A M
Abstract excerpt
We isolated and treated in vitro with a novel CD5-specific saporin immunotoxin, referred to as OKT1-SAP, the cells infiltrating an irreversibly rejected renal allograft from a patient who rejected while on cyclosporine plus steroids and then failed to respond to multiple courses of high-dose steroids, intravenous OKT3 antibody, and local irradiation to the graft. We report here that under experimental conditions...
Topics
- Adult
- Antibodies, Monoclonal
- Antigens, Differentiation
- CD5 Antigens
- Cell Separation
- Cells, Cultured
- Flow Cytometry
- Fluorescent Antibody Technique
- Graft Rejection
- Humans