Article
[The expression of KATP; channel mutant subunit Kir6.2AAA in rat cardiomyocytes].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 1 Apr 2014
Zhang Xiaohui, Wei Chunshan, Lin Jian, Xiong Yiqun, Xu Chaoying, Liu Xinliang, Mu Guiping, Xu Shaogang, Liu Wenhe
Abstract excerpt
OBJECTIVE: To construct a recombinant adenovirus vector carrying KATP; channel mutant subunit Kir6.2AAA and express it in rat cardiomyocytes. METHODS: Based on the primers for Kir6.2 subunits, Kir6.2 GFG amino acids were site-directed mutated into AAA by means of overlap PCR. PCR products were cloned into pShuttle vector for sequence analysis. After Pme I linearization, it was transformed into adenovirus...
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