[Construction of expression vectors for C-terminally deleted NR2B subunit mutants and their application in the study of assembling of NMDA receptors].

doi:10.3785/j.issn.1008-9292.2003.06.005

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Article

[Construction of expression vectors for C-terminally deleted NR2B subunit mutants and their application in the study of assembling of NMDA receptors].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences - 1 Dec 2003

Yang Wei, Luo Jian-hong, Gao Ying, Huang Man, Zheng Chan-ying

Abstract excerpt

OBJECTIVE: To investigate the role of NR2B subunit C-terminus in assembling and surface expression of NMDA receptor subtype composed of NR1-1a/NR2B subunits. METHODS: Eight vectors NR2BDelta1-Delta8) expressing GFP-tagged NR2B subunit mutants with various deletion in the carboxyl-terminal region were generated by conventional molecular cloning techniques. Each of these vectors was transfected alone, or...

Topics

Cell Line
Genetic Vectors
Humans
Mutation
Receptors, N-Methyl-D-Aspartate
Transfection

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Open in PubMed

Identifiers

PMID: 14712509
DOI: 10.3785/j.issn.1008-9292.2003.06.005

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