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Article

Rapid visual detection of hepatitis C virus using a reverse transcription loop-mediated isothermal amplification assay.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases - 1 Jan 2021

Hongjaisee Sayamon, Doungjinda Natteewan, Khamduang Woottichai, Carraway Tanawan Samleerat, Wipasa Jiraprapa, Debes Jose D, Supparatpinyo Khuanchai

Abstract excerpt

OBJECTIVES: The aim was to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of hepatitis C virus (HCV) in a single closed tube. METHODS: Plasma samples were collected from 200 HCV-infected patients. HCV-RNA was detected by one-step RT-LAMP processed at 65 °C for 60 min. The amplified products were detected by hydroxynaphthol blue (HNB)-dependent visual...

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Identifiers

PMID: 33130211
DOI: 10.1016/j.ijid.2020.10.082
PMCID: PMC7794100

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