Article
An improved Protein G with higher affinity for human/rabbit IgG Fc domains exploiting a computationally designed polar network.
Protein engineering, design & selection : PEDS - 1 Apr 2014
Jha Ramesh K, Gaiotto Tiziano, Bradbury Andrew R M, Strauss Charlie E M
Abstract excerpt
Protein G is an IgG binding protein that has been widely exploited for biotechnological purposes. Rosetta protein modeling identified a set of favorable polar mutations in Protein G, at its binding interface with the Fc domain of Immunoglobulin G, that were predicted to increase the stability and tighten the binding relative to native Protein G, with only a minor perturbation of the binding mode seen in the...
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