Article
X-ray microfluorescence (μXRF) imaging of Aspergillus nidulans cell wall mutants reveals biochemical changes due to gene deletions.
Analytical and bioanalytical chemistry - 1 May 2014
Rak Margaret, Salome Murielle, Kaminskyj Susan G W, Gough Kathleen M
Abstract excerpt
We used synchrotron X-ray fluorescence to create the first semiquantitative, submicron resolution, element distribution maps of P, S, K, and Ca, in situ, in fungal samples. Data collection was performed at the European Synchrotron Radiation Facility beam line ID21, Grenoble, France. We studied developing hyphae, septa, and conidiophores in Aspergillus nidulans, comparing wild type and two cell wall biosynthesis...
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