Article
Serum- and growth-factor-free three-dimensional culture system supports cartilage tissue formation by promoting collagen synthesis via Sox9-Col2a1 interaction.
Tissue engineering. Part A - 1 Aug 2014
Ahmed Nazish, Iu Jonathan, Brown Chelsea E, Taylor Drew Wesley, Kandel Rita A
Abstract excerpt
OBJECTIVE: One of the factors preventing clinical application of regenerative medicine to degenerative cartilage diseases is a suitable source of cells. Chondrocytes, the only cell type of cartilage, grown in vitro under culture conditions to expand cell numbers lose their phenotype along with the ability to generate hyaline cartilaginous tissue. In this study we determine that a serum- and growth-factor-free...
Topics
- Animals
- Cartilage, Articular
- Cattle
- Cell Culture Techniques
- Cell Differentiation
- Cells, Cultured
- Chondrocytes
- Chondrogenesis
- Collagen Type II
- Culture Media, Serum-Free
- Dexamethasone
- Insulin
- Intercellular Signaling Peptides and Proteins