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Establishment and Maintenance of Human Embryonic Stem Cells on STO, a Permanently Growing Cell Line1

1 Dec 2003

Abstract excerpt

Human embryonic stem (hES) cells have been traditionally cultured on primary mouse embryonic fibroblasts (PMEFs). However, though STO cells have some advantages over PMEFs and human embryonic fibroblasts (hEFs) as feeder cells, they have never been used as feeder cells to establish hES cell lines. In this study, three hES cell lines (Miz-hES1, Miz-hES2, and Miz-hES3) were established from inner cell masses (ICM),...

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PMID: 12930726
DOI: 10.1095/biolreprod.103.017467

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