Article
SILAC-based proteomic quantification of chemoattractant-induced cytoskeleton dynamics on a second to minute timescale.
Nature communications - 26 Feb 2014
Sobczyk Grzegorz J, Wang Jun, Weijer Cornelis J
Abstract excerpt
Cytoskeletal dynamics during cell behaviours ranging from endocytosis and exocytosis to cell division and movement is controlled by a complex network of signalling pathways, the full details of which are as yet unresolved. Here we show that SILAC-based proteomic methods can be used to characterize the rapid chemoattractant-induced dynamic changes in the actin-myosin cytoskeleton and regulatory elements on a...
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