Article
Optimization of standard in-house 24-locus variable-number tandem-repeat typing for Mycobacterium tuberculosis and its direct application to clinical material.
Journal of clinical microbiology - 1 May 2014
de Beer Jessica L, Akkerman Onno W, Schürch Anita C, Mulder Arnout, van der Werf Tjip S, van der Zanden Adri G M, van Ingen Jakko, van Soolingen Dick
Abstract excerpt
Variable-number tandem-repeat (VNTR) typing with a panel of 24 loci is the current gold standard in the molecular typing of Mycobacterium tuberculosis complex isolates. However, because of technical problems, a part of the loci often cannot be amplified by multiplex PCRs. Therefore, a considerable number of single-locus PCRs have to be performed for the loci with missing results, which impairs the laboratory work...
Topics
- DNA Primers
- Genetic Loci
- Genotype
- Molecular Typing
- Mycobacterium tuberculosis
- Polymerase Chain Reaction
- Tandem Repeat Sequences