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Article

Utility and evaluation of new variable-number tandem-repeat systems for genotyping mycobacterial tuberculosis isolates.

Journal of microbiological methods - 1 Apr 2009

Dou Horng-Yunn, Lu Jang-Jih, Lin Chih-Wei, Chang Jia-Ru, Sun Jun-Ren, Su Ih-Jen

Abstract excerpt

We compared mycobacterial interspersed repetitive unit (MIRU)-variable number tandem repeat (VNTR) typing to traditional spoligotyping for discriminating Mycobacterium tuberculosis (MTB) strains. Our 17-loci MIRU-VNTR typing method was found to be superior to spoligotyping for non-Beijing family strains. To extend the method we also established PCR-based rapid genotyping protocols for Beijing, East-African-Indian...

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PMID: 19386225
DOI: 10.1016/j.mimet.2009.01.007

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