Article
Screening for imprinted genes using high-resolution melting analysis of PCR amplicons.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Day Robert, Macknight Richard
Abstract excerpt
High-resolution melting (HRM) analysis is a technique that enables researchers to detect polymorphisms in DNA molecules based on different melting profiles and is becoming widely used as a method for detecting SNPs in genomic DNA. In this chapter, we describe how HRM analysis can be used to detect allelic imbalances typical of imprinted genes, where alleles are differentially expressed based on their parent of...
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