Article
Antisense oligodeoxynucleotide inhibition as an alternative and convenient method for gene function analysis in pollen tubes.
PloS one - 1 Jan 2013
Liao Fanglei, Wang Lu, Yang Li-Bo, Zhang Liyao, Peng Xiongbo, Sun Meng-Xiang
Abstract excerpt
Antisense oligodeoxynucleotide (A-ODN) inhibition works well in animal cells. However, there have been few successful examples to date of its application in plants, and more specifically whether the technique can be used in pollen tubes as a model of plant cell growth. NtGNL1 plays an important role in pollen tube development and was thus selected as an indicator to assess the biological effects of A-ODN. An...
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