Article
Primer effect in the detection of mtDNA heteroplasmy: insights from horse Cytochrome b gene.
Mitochondrial DNA - 1 Apr 2015
Zhao Qianjun, Kang Ye, Pu Yabin, Niu Lili, Guan Weijun, He Xiaohong, Zhang Hongping, Lim Hyuntae, Ma Yuehui, Zhong Tao
Abstract excerpt
Heteroplasmy, the presence of bi-allelic mtDNA types within an individual, has been previously detected in the D-loop region and Cytochrome b gene (Cytb) of mitochondrial DNA using PCR-RFLP. However, heteroplasmy was absent in thousands of equine mtDNA sequences deposited in GenBank. To address whether heteroplasmy widely exists in mitochondria of Chinese indigenous horses, we generated the data set of the target...
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