Article
Sequencing strategy of mitochondrial HV1 and HV2 DNA with length heteroplasmy.
Forensic science international - 9 Oct 2002
Rasmussen E M, Sørensen E, Eriksen B, Larsen H J, Morling N
Abstract excerpt
We describe a method to obtain reliable mitochondrial DNA (mtDNA) sequences downstream of the homopolymeric stretches with length heteroplasmy in the sequencing direction. The method is based on the use of junction primers that bind to a part of the homopolymeric stretch and the first 2-4 bases downstream of the homopolymeric region. This junction primer method gave clear and unambiguous results using samples...
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