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Article

Single molecule activity measurements of cytochrome P450 oxidoreductase reveal the existence of two discrete functional states.

ACS chemical biology - 21 Mar 2014

Laursen Tomas, Singha Aparajita, Rantzau Nicolai, Tutkus Marijonas, Borch Jonas, Hedegård Per, Stamou Dimitrios, Møller Birger Lindberg, Hatzakis Nikos S

Abstract excerpt

Electron transfer between membrane spanning oxidoreductase enzymes controls vital metabolic processes. Here we studied for the first time with single molecule resolution the function of P450 oxidoreductase (POR), the canonical membrane spanning activator of all microsomal cytochrome P450 enzymes. Measurements and statistical analysis of individual catalytic turnover cycles shows POR to sample at least two major...

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PMID: 24359083
DOI: 10.1021/cb400708v

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POR

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