Article
MDR1 synonymous polymorphisms alter transporter specificity and protein stability in a stable epithelial monolayer.
Cancer research - 15 Jan 2014
Fung King Leung, Pan James, Ohnuma Shinobu, Lund Paul E, Pixley Jessica N, Kimchi-Sarfaty Chava, Ambudkar Suresh V, Gottesman Michael M
Abstract excerpt
The drug efflux function of P-glycoprotein (P-gp) encoded by MDR1 can be influenced by genetic polymorphisms, including two synonymous changes in the coding region of MDR1. Here we report that the conformation of P-gp and its drug efflux activity can be altered by synonymous polymorphisms in stable epithelial monolayers expressing P-gp. Several cell lines with similar MDR1 DNA copy number were developed and...
Topics
- ATP Binding Cassette Transporter, Subfamily B
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Adenosine Triphosphatases
- Animals
- Biological Transport
- Biotinylation
- Cell Line
- Cell Line, Tumor
- Cell Proliferation
- Drug Resistance, Neoplasm
- Gene Expression Regulation, Neoplastic