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Article

A His6-SUMO-eXact tag for producing human prepro-urocortin 2 in Escherichia coli for raising monoclonal antibodies.

Journal of immunological methods - 31 Jan 2014

Liew Oi Wah, Ang Cui Xia, Peh Yu Pei, Chong Pek Ching Jenny, Ng Yan Xia, Hwang Le-Ann, Koh Xin Yu, Yip Yin Mun, Liu Wei, Richards A Mark

Abstract excerpt

This is a first report of recombinant production of human prepro-Urocortin 2 in Escherichia coli by N-terminal fusion with a triple His₆-SUMO-eXact tag and its subsequent use as an antigen for the production and screening of very high affinity monoclonal antibodies. The rationale for this combina...

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Source

Identifiers

PMID: 24291344
DOI: 10.1016/j.jim.2013.11.015

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-24291344-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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