Understanding the mechanism of prosegment-catalyzed folding by solution NMR spectroscopy.

Abstract excerpt

Multidomain protein folding is often more complex than a two-state process, which leads to the spontaneous folding of the native state. Pepsin, a zymogen-derived enzyme, without its prosegment (PS), is irreversibly denatured and folds to a thermodynamically stable, non-native conformation, termed refolded pepsin, which is separated from native pepsin by a large activation barrier. While it is known that PS binds...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Catalysis
• Share to Catalytic Domain
• Share to Crystallography, X-Ray