Article
Induction of competent cells for Agrobacterium tumefaciens-mediated stable transformation of common bean (Phaseolus vulgaris L.).
PloS one - 1 Jan 2020
Song Guo-Qing, Han Xue, Wiersma Andrew T, Zong Xiaojuan, Awale Halima E, Kelly James D
Abstract excerpt
Stable transformation of common bean (Phaseolus vulgaris L.) has been successful, to date, only using biolistic-mediated transformation and shoot regeneration from meristem-containing embryo axes. In this study, using precultured embryo axes, and optimal co-cultivation conditions resulted in a successful transformation of the common bean cultivar Olathe using Agrobacterium tumefaciens strain EHA105. Plant...
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