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Article

Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method.

Planta - 1 Mar 2003

Olhoft Paula M, Flagel Lex E, Donovan Christopher M, Somers David A

Abstract excerpt

The efficiency of soybean [Glycine max (L.) Merrill] transformation was significantly increased from an average of 0.7% to 16.4% by combining strategies to enhance Agrobacterium tumefaciens-mediated T-DNA delivery into cotyledonary-node cells with the development of a rapid, efficient selection protocol based on hygromycin B. Wounded cotyledonary-node explants were inoculated with A. tumefaciens carrying either a...

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PMID: 12624759
DOI: 10.1007/s00425-002-0922-2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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