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Article

Doubled Haploid Transgenic Wheat Lines by Microspore Transformation.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Rustgi Sachin, Ankrah Nii O, Brew-Appiah Rhoda A T, Sun Yue, Liu Weiguo, von Wettstein Diter

Abstract excerpt

Microspores are preferred explant choice for genetic transformation, as their use shortens the duration of obtaining homozygous transformants. All established gene-delivery methods of particle bombardment, electroporation, and cocultivation with Agrobacterium tumefaciens were optimized on androgenic microspores or derived tissues. In the biolistic gene delivery method 35-40 days old haploid microspore embryoids...

Topics

Agrobacterium tumefaciens
Biolistics
Cell Culture Techniques
Electroporation
Gene Transfer Techniques
Genetic Vectors
Haploidy
Phenotype
Pollen
Transformation, Genetic
Triticum

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 28913803
DOI: 10.1007/978-1-4939-7337-8_13

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