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Article

Doubled Haploid Transgenic Wheat Lines by Microspore Transformation.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Rustgi Sachin, Ankrah Nii O, Brew-Appiah Rhoda A T, Sun Yue, Liu Weiguo, von Wettstein Diter

Abstract excerpt

Microspores are preferred explant choice for genetic transformation, as their use shortens the duration of obtaining homozygous transformants. All established gene-delivery methods of particle bombardment, electroporation, and cocultivation with Agrobacterium tumefaciens were optimized on androgenic microspores or derived tissues. In the biolistic gene delivery method 35-40 days old haploid microspore embryoids...

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PMID: 28913803
DOI: 10.1007/978-1-4939-7337-8_13

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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