Article
High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens.
Nature biotechnology - 1 Jun 1996
Ishida Y, Saito H, Ohta S, Hiei Y, Komari T, Kumashiro T
Abstract excerpt
Transformants of maize inbred A188 were efficiently produced from immature embryos cocultivated with Agrobacterium tumefaciens that carried "super-binary" vectors. Frequencies of transformation (independent transgenic plants/embryos) were between 5% and 30%. Almost all transformants were normal in morphology, and more than 70% were fertile. Stable integration, expression, and inheritance of the transgenes were...
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