High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens.

doi:10.1038/nbt0696-745

Back to search

Article

High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens.

Nature biotechnology - 1 Jun 1996

Ishida Y, Saito H, Ohta S, Hiei Y, Komari T, Kumashiro T

Abstract excerpt

Transformants of maize inbred A188 were efficiently produced from immature embryos cocultivated with Agrobacterium tumefaciens that carried "super-binary" vectors. Frequencies of transformation (independent transgenic plants/embryos) were between 5% and 30%. Almost all transformants were normal i...

Topics

Agrobacterium tumefaciens
Base Sequence
Culture Techniques
DNA, Bacterial
Genetic Markers
Genetic Vectors
Genotype
Molecular Sequence Data
Seeds
Transformation, Genetic
Zea mays

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.