Genomic subtraction for cloning DNA corresponding to deletion mutations.

doi:10.1073/pnas.87.5.1889

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Article

Genomic subtraction for cloning DNA corresponding to deletion mutations.

Proceedings of the National Academy of Sciences of the United States of America - 1 Mar 1990

Straus D, Ausubel F M

Abstract excerpt

We have developed a technique, called genomic subtraction, for isolating the DNA that is absent in deletion mutants. The method removes from wild-type DNA the sequences that are present in both the wild-type and the deletion mutant genomes. The DNA that corresponds to the deleted region remains....

Topics

Affinity Labels
Azides
Base Sequence
Biotin
Chromosome Deletion
Cloning, Molecular
DNA, Fungal
Molecular Sequence Data
Mutation
Oligonucleotide Probes
Plasmids
Polymerase Chain Reaction
Saccharomyces cerevisiae

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