Article
Subtraction hybridisation and shot-gun sequencing: a new approach to identify symbiotic loci.
Nucleic acids research - 25 Apr 1994
Perret X, Fellay R, Bjourson A J, Cooper J E, Brenner S, Broughton W J
Abstract excerpt
Traditionally, new loci involved in the Rhizobium-legume symbiosis have been identified by transposon mutagenesis and/or complementation. Wide dispersal of the symbiotic loci in Rhizobium species NGR234, as well as the large number of potential host-plants to be screened, greatly reduces the efficiency of these techniques. As an alternate strategy designed to identify new NGR234 genes involved in the early stages...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Fabaceae
- Genetic Complementation Test
- Molecular Sequence Data
- Mutagenesis, Insertional
- Nucleic Acid Hybridization