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Article

Subtraction hybridisation and shot-gun sequencing: a new approach to identify symbiotic loci.

Nucleic acids research - 25 Apr 1994

Perret X, Fellay R, Bjourson A J, Cooper J E, Brenner S, Broughton W J

Abstract excerpt

Traditionally, new loci involved in the Rhizobium-legume symbiosis have been identified by transposon mutagenesis and/or complementation. Wide dispersal of the symbiotic loci in Rhizobium species NGR234, as well as the large number of potential host-plants to be screened, greatly reduces the efficiency of these techniques. As an alternate strategy designed to identify new NGR234 genes involved in the early stages...

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Identifiers

PMID: 8190622
DOI: 10.1093/nar/22.8.1335
PMCID: PMC307986

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