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Screening transposon mutant libraries using full-genome oligonucleotide microarrays.

Methods in enzymology - 1 Jan 2007

Winterberg Kelly M, Reznikoff William S

Abstract excerpt

The experimental details for a high-throughput microarray-based screening technique for both detecting and mapping Tn5 insertion mutants in parallel within a library are presented. Following Tn5 mutagenesis, viable mutants are pooled and grown competitively under selective conditions. Chromosomal DNA is then isolated from each mutant pool. Biotin-labeled run-off in vitro RNA transcripts, representing the...

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PMID: 17352919
DOI: 10.1016/S0076-6879(06)21011-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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