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Article

Identification of a mutant locus by noncomplementation of a transposon insertion library in Saccharomyces cerevisiae.

Genetics - 1 Aug 2001

Wiatrowski H A, Carlson M

Abstract excerpt

We describe a new approach for identifying the gene corresponding to a mutation in Saccharomyces cerevisiae. A library of mTn-lacZ/LEU2 insertions is tested for failure to complement the mutation, and the noncomplementing insertion is used to obtain sequence. This approach offers an alternative to cloning by complementation with a plasmid library.

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Identifiers

PMID: 11514465
DOI: 10.1093/genetics/158.4.1825
PMCID: PMC1461749

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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