Article
Activation of Ran GTPase by a Legionella effector promotes microtubule polymerization, pathogen vacuole motility and infection.
PLoS pathogens - 1 Sept 2013
Rothmeier Eva, Pfaffinger Gudrun, Hoffmann Christine, Harrison Christopher F, Grabmayr Heinrich, Repnik Urska, Hannemann Mandy, Wölke Stefan, Bausch Andreas, Griffiths Gareth, Müller-Taubenberger Annette, Itzen Aymelt, Hilbi Hubert
Abstract excerpt
The causative agent of Legionnaires' disease, Legionella pneumophila, uses the Icm/Dot type IV secretion system (T4SS) to form in phagocytes a distinct "Legionella-containing vacuole" (LCV), which intercepts endosomal and secretory vesicle trafficking. Proteomics revealed the presence of the small GTPase Ran and its effector RanBP1 on purified LCVs. Here we validate that Ran and RanBP1 localize to LCVs and...
Topics
- Animals
- Bacterial Proteins
- Cell Line
- Enzyme Activation
- GTPase-Activating Proteins
- Gene Silencing
- Humans
- Legionella pneumophila
- Legionnaires' Disease
- Macrophages