Characterization of the double mutant, Val-177/Asn-322, of the lactose permease.

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Article

Characterization of the double mutant, Val-177/Asn-322, of the lactose permease.

The Journal of biological chemistry - 5 Mar 1990

Brooker R J

Abstract excerpt

The double mutant, Val-177/Asn-322, was investigated with regard to its ability to transport H+ and galactosides. In downhill lactose transport assays, the wild-type strain had a Km value for lactose uptake of 0.9 mM and a Vmax of 0.65 mumol lactose/min.mg protein while the mutant had a significa...

Topics

Asparagine
DNA, Bacterial
Drug Combinations
Escherichia coli
Escherichia coli Proteins
Hydrogen-Ion Concentration
Kinetics
Lactose
Membrane Transport Proteins
Methylgalactosides
Monosaccharide Transport Proteins
Mutation
Plasmids
Symporters
Thiogalactosides
Valine

Source

Open in PubMed

Identifiers

PMID: 2406272

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2406272-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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