Article
Evidence that the asparagine 322 mutant of the lactose permease transports protons and lactose with a normal stoichiometry and accumulates lactose against a concentration gradient.
The Journal of biological chemistry - 15 Apr 1991
Franco P J, Brooker R J
Abstract excerpt
The single asparagine 322 mutant of the lactose permease was made by constructing a hybrid plasmid which contained the amino-terminal coding sequence from the wild-type permease gene and the carboxyl-terminal coding sequence from a previously characterized double mutant permease which contained a...
Topics
- Asparagine
- Escherichia coli
- Escherichia coli Proteins
- Genotype
- Hydrogen-Ion Concentration
- Kinetics
- Lactose
- Membrane Transport Proteins
- Monosaccharide Transport Proteins
- Mutation
- Plasmids
- Protons
- Restriction Mapping
- Substrate Specificity
- Symporters