Article
Identification of valine 177 as a mutation altering specificity for transport of sugars by the Escherichia coli lactose carrier. Enhanced specificity for sucrose and maltose.
The Journal of biological chemistry - 15 Jun 1990
King S C, Wilson T H
Abstract excerpt
A mutant of the Escherichia coli lactose carrier has been selected (in an invertase-positive strain) based on its ability to grow on 6 mM sucrose in a manner dependent upon lactose carrier induction by isopropyl-1-thio-beta-D-galactopyranoside. The mutant was cloned, and DNA sequencing revealed a...
Topics
- Biological Transport
- DNA, Bacterial
- Escherichia coli
- Escherichia coli Proteins
- Genotype
- Kinetics
- Maltose
- Membrane Transport Proteins
- Monosaccharide Transport Proteins
- Mutation
- Plasmids
- Substrate Specificity
- Sucrose
- Symporters
- Valine